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40 Hz vibrations reduce Alzheimer’s pathology, symptoms in mouse models

Tactile stimulation improved motor performance, reduced phosphorylated tau, preserved neurons and synapses, and reduced DNA damage, a new study shows.
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Two panels show a section of mouse brain tissue with a dull magenta glow over a black background. In the left panel many neurons are lit up brightly in magenta. In the right panel only one neuron is lit up brightly.
A new study presents evidence that 40 Hz vibration can reduce Alzheimer's disease pathology and symptoms in lab mice and improve their motor function. These images highlight reductions in the hallmark Alzheimer's disease protein phosphorylated tau (magenta) in primary somatosensory cortical neurons in Tau P301S model mice treated with 40 Hz tactile stimulation (right). An image from an untreated control is on the left.
Image: Tsai Lab/The Picower Institute

Evidence that noninvasive sensory stimulation of 40 Hz gamma frequency brain rhythms can reduce Alzheimer’s disease pathology and symptoms, already shown with light and sound by multiple research groups in mice and humans, now extends to tactile stimulation. A new study by MIT scientists shows that Alzheimer’s model mice exposed to 40 Hz vibration for an hour a day for several weeks showed improved brain health and motor function compared to untreated controls.

The MIT group is not the first to show that gamma frequency tactile stimulation can affect brain activity and improve motor function, but they are the first to show that the stimulation can also reduce levels of the hallmark Alzheimer’s protein phosphorylated tau, keep neurons from dying or losing their synapse circuit connections, and reduce neural DNA damage.

“This work demonstrates a third sensory modality that we can use to increase gamma power in the brain,” says Li-Huei Tsai, corresponding author of the study, director of The Picower Institute for Learning and Memory and the Aging Brain Initiative at MIT, and Picower Professor in the Department of Brain and Cognitive Sciences (BCS). “We are very excited to see that 40 Hz tactile stimulation benefits motor abilities, which has not been shown with the other modalities. It would be interesting to see if tactile stimulation can benefit human subjects with impairment in motor function.”

Ho-Jun Suk, Nicole Buie, Guojie Xu, and Arit Banerjee are lead authors of the study in Frontiers in Aging Neuroscience, and Ed Boyden, the Y. Eva Tan Professor of Neurotechnology at MIT, is a co-senior author of the paper. Boyden, an affiliate member of The Picower Institute, is also appointed in BCS as well as the departments of Biological Engineering and Media Arts and Sciences, the McGovern Institute for Brain Research, and the K. Lisa Yang Center for Bionics.

Feeling the vibe

In a series of papers starting in 2016, a collaboration led by Tsai’s lab has demonstrated that light flickering and/or sound clicking at 40 Hz (a technology called GENUS for Gamma Entrainment Using Sensory Stimuli), reduces levels of amyloid-beta and tau proteins, prevents neuron death, and preserves synapses and even sustains learning and memory in a variety of Alzheimer’s disease mouse models. Most recently, in pilot clinical studies the team showed that 40 Hz light and sound stimulation was safe, successfully increased brain activity and connectivity, and appeared to produce significant clinical benefits in a small cohort of human volunteers with early-stage Alzheimer’s disease. Other groups have replicated and corroborated health benefits of 40 Hz sensory stimulation, and an MIT spinoff company, Cognito Therapeutics, has launched stage III clinical trials of light and sound stimulation as an Alzheimer’s treatment.

The new study tested whether whole-body 40 Hz tactile stimulation produced meaningful benefits in two commonly used mouse models of Alzheimer’s neurodegeneration, the Tau P301S mouse, which recapitulates the disease’s tau pathology, and the CK-p25 mouse, which recapitulates the synapse loss and DNA damage seen in human disease. The team focused its analyses in two areas of the brain: the primary somatosensory cortex (SSp), where tactile sensations are processed, and the primary motor cortex (MOp), where the brain produces movement commands for the body.

To produce the vibration stimulation, the researchers placed mouse cages over speakers playing 40 Hz sound, which vibrated the cages. Non-stimulated control mice were in cages interspersed in the same room so that all the mice heard the same 40 Hz sound. The differences measured between the stimulated and control mice were therefore made by the addition of tactile stimulation.

First the researchers confirmed that 40 Hz vibration made a difference in neural activity in the brains of healthy (i.e., non-Alzheimer’s) mice. As measured by expression of c-fos protein, activity increased two-fold in the SSp and more than three-fold in the MOp, a statistically significant increase in the latter case.

Once the researchers knew that 40 Hz tactile stimulation could increase neural activity, they assessed the impact on disease in the two mouse models. To ensure both sexes were represented, the team used male P301S mice and female CK-p25 mice.

Good vibrations

P301S mice stimulated for three weeks showed significant preservation of neurons compared to unstimulated controls in both brain regions. Stimulated mice also showed significant reductions in tau in the SSp by two measures, and exhibited similar trends in the MOp.

CK-p25 mice received six weeks of vibration stimulation. These mice showed higher levels of synaptic protein markers in both brain regions compared to unvibrated control mice. They also showed reduced levels of DNA damage.

Finally, the team assessed the motor abilities of mice exposed to the vibration versus not-exposed. They found that both mouse models were able to stay on a rotating rod significantly longer. P301S mice also hung onto a wire mesh for significantly longer than control mice, while CK-p25 mice showed a positive, though non-significant, trend.

“The current study, along with our previous studies using visual or auditory GENUS, demonstrates the possibility of using noninvasive sensory stimulation as a novel therapeutic strategy for ameliorating pathology and improving behavioral performance in neurodegenerative diseases,” the authors conclude.

Support for the study came from The Picower Institute for Learning and Memory, The JPB Foundation, Halis Family Foundation, Eduardo Eurnekian, DeGroof-VM Foundation, The Dolby Family, Melissa Ko Hahn and Suguwu Douglas Hahn, Lester A. Gimpelson, Kathleen and Miguel Octavio, Glenda G. and Donald A. Mattes, Carol and Gene Ludwig Family Foundation, Eleanor Schwartz Charitable Foundation, David B. Emmes, Jay L. and Carroll Miller, Alan and Susan Patricof, Alan Alda, Willian C.L. and Bernadette Shih, Eduardo Bross, Anne Gao and Alex Hu, Prisca Chen Marvin and Kim A. Marvin., and Charles Hieken.

Press Mentions


Cognito Therapeutics, founded by Prof. Ed Boyden and Prof. Li Huei Tsai, has developed a “specialized headset that delivers 40Hz auditory and visual stimulation” to the brain, which could potentially slow down the cognitive decline and neurodegeneration in Alzheimer’s disease, reports William A. Haseltine for Forbes. Prof. Li-Huei Tsai “and her team speculated that if gamma wave activity is reduced in Alzheimer’s disease, perhaps, artificially stimulating the brain may enhance synchronized firing and restore cognition,” writes Haseltine.


Cognito Therapeutics, founded by Prof. Ed Boyden and Prof. Li-Huei Tsai, is using a 40 Hzlight-flickering and auditory headset to help slow the progression of Alzheimer’s and restore cognition, reports William A. Haseltine for Forbes. “A recent pilot clinical trial found that this technology is not only safe and tolerable for home use, but also has a positive impact on reducing symptoms associated with age-related neurodegeneration,” writes Haseltine.

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